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Flexible material formulations for 3D printing of ordered porous beds with applications in bioprocess engineering

Dimartino, Simone; Galindo-Rodriguez, Giuseppe Rafael; Simon, Ursula; Conti, Mariachiara; Sarwar, M. Sulaiman; Athi Narayanan, Selva Manikandan; Jiang, Qihao; Christofi, Nick

Authors

Simone Dimartino

Giuseppe Rafael Galindo-Rodriguez

Ursula Simon

Mariachiara Conti

M. Sulaiman Sarwar

Selva Manikandan Athi Narayanan

Qihao Jiang

Nick Christofi



Abstract

Background
3D printing is revolutioning many industrial sectors and has the potential to enhance also the biotechnology and bioprocessing fields. Here, we propose a new flexible material formulation to 3D print support matrices with complex, perfectly ordered morphology and with tuneable properties to suit a range of applications in bioprocess engineering.

Findings
Supports were fabricated using functional monomers as the key ingredients, enabling matrices with bespoke chemistry, such as charged groups, chemical moieties for further functionalization, and hydrophobic/hydrophilic groups. Other ingredients, e.g. crosslinkers and porogens, can be employed to fabricate supports with diverse characteristics of their porous network, providing an opportunity to further regulate the mechanical and mass transfer properties of the supports. Through this approach, we fabricated and demonstrated the operation of Schoen gyroid columns with (I) positive and negative charges for ion exchange chromatography, (II) enzyme bioreactors with immobilized trypsin to catalyse hydrolysis, and (III) bacterial biofilm bioreactors for fuel desulphurization.

Conclusions
This study demonstrates a simple, cost-effective, and flexible fabrication of customized 3D printed supports for different biotechnology and bioengineering applications.

Journal Article Type Article
Acceptance Date Feb 22, 2022
Online Publication Date Mar 12, 2022
Publication Date 2022
Deposit Date Mar 31, 2022
Publicly Available Date Mar 31, 2022
Journal Bioresources and Bioprocessing
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 9
Issue 1
Article Number 20
DOI https://doi.org/10.1186/s40643-022-00511-9
Keywords Biomaterials, Additive manufacturing, Chromatography, Immobilized enzyme bioreactor, Bacterial biofilm bioreactor
Public URL http://researchrepository.napier.ac.uk/Output/2859662

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