Spread of a single multiresistant methicillin-resistant Staphylococcus aureus clone carrying a variant of staphylococcal cassette chromosome mec type III isolated in a university hospital

Szczepanik, A.; Kozio?-Montewka, M.; Al-Doori, Z.; Morrison, D.; Kaczor, D.

Abstract

The purpose of the study was the molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolates cultured from patients treated in seven wards of a university hospital in Lublin, Poland, over a 14-month period. Eleven nosocomial MRSA isolates were analyzed. Phenotypic identification of the isolates as MRSA was confirmed by the detection of the nuc and mecA genes using a multiplex PCR assay. The MRSA isolates were further characterized by pulsed-field gel electrophoresis, 16S-23S rRNA spacer length polymorphism analysis, and the simplex and multiplex SCCmec PCR assays. The MRSA isolates were found to be multiresistant: in addition to resistance to β-lactam agents, they demonstrated resistance to ciprofloxacin, tetracycline, erythromycin, and gentamicin. The MRSA isolates were genetically identical and shared common pulsed-field gel electrophoresis profiles and 16S-23S rRNA spacer length polymorphism profiles. The PCR-based method revealed that the profile of the Lublin clone was identical to that of the Brazilian pandemic MRSA isolates. By SCCmec typing, all MRSA isolates harbored the C variant of the SCCmec type III that differed from the typical SCCmec type III pattern by the lack of locus F (414 bp). The results of this study indicate the spread of a single, multiresistant, MRSA clone in various wards of a university hospital over a 14-month period. The SCCmec structure harbored by the Lublin clone has previously been identified among Polish MRSA isolates representing the HoMRSA-Pol1 clone. The data from this study indicate that the Lublin MRSA clone is most probably genetically related to the HoMRSA-Pol1 clone. Moreover, this latter clone belongs to ST239, the same sequence type as the Hungarian and Brazilian pandemic MRSA isolates.

Citation

Szczepanik, A., Kozioł-Montewka, M., Al-Doori, Z., Morrison, D., & Kaczor, D. (2007). Spread of a single multiresistant methicillin-resistant Staphylococcus aureus clone carrying a variant of staphylococcal cassette chromosome mec type III isolated in a university hospital. European Journal of Clinical Microbiology and Infectious Diseases, 26(1), 29-35. https://doi.org/10.1007/s10096-006-0237-5

• Journal Article Type: Article
• Online Publication Date: Dec 19, 2006
• Publication Date: Jan 9, 2007
• Deposit Date: May 1, 2020
• Journal: European Journal of Clinical Microbiology & Infectious Diseases
• Print ISSN: 0934-9723
• Electronic ISSN: 1435-4373
• Publisher: Springer
• Peer Reviewed: Peer Reviewed
• Volume: 26
• Issue: 1
• Pages: 29-35
• DOI: https://doi.org/10.1007/s10096-006-0237-5
• Public URL: http://researchrepository.napier.ac.uk/Output/2657683