Hippocalcin functions as a calcium sensor in hippocampal LTD

Palmer, Claire; Lim, Wonil; Hastie, Peter G R; Toward, Mark; Korolchuk, Victor I; Burbidge, Stephen A; Banting, George; Collingridge, Graham L; Isaac, John T R; Henley, Jeremy M

Abstract

It is not fully understood how NMDAR-dependent LTD causes Ca2+-dependent endocytosis of AMPARs. Here we show that the neuronal Ca2+ sensor hippocalcin binds the β2-adaptin subunit of the AP2 adaptor complex and that along with GluR2 these coimmunoprecipitate in a Ca2+-sensitive manner. Infusion of a truncated mutant of hippocalcin (HIP2-72) that lacks the Ca2+ binding domains prevents synaptically evoked LTD but has no effect on LTP. These data indicate that the AP2-hippocalcin complex acts as a Ca2+ sensor that couples NMDAR-dependent activation to regulated endocytosis of AMPARs during LTD.

Citation

Palmer, C., Lim, W., Hastie, P. G. R., Toward, M., Korolchuk, V. I., Burbidge, S. A., Banting, G., Collingridge, G. L., Isaac, J. T. R., & Henley, J. M. (2005). Hippocalcin functions as a calcium sensor in hippocampal LTD. Neuron, 47(4), 487-494. https://doi.org/10.1016/j.neuron.2005.06.014

• Journal Article Type: Article
• Publication Date: 2005-08
• Deposit Date: Jun 24, 2008
• Print ISSN: 0896-6273
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 47
• Issue: 4
• Pages: 487-494
• DOI: https://doi.org/10.1016/j.neuron.2005.06.014
• Keywords: AMPARs; Hippocalcin; NMDAR-dependent LTDs
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/1655
• Publisher URL: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSS-4GWS8KH-6&_user=132448&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000011019&_version=1&_urlVersion=0&_userid=132448&md5=49c4d0465373ec20c6a74c56ccc680c4