Hippocalcin functions as a calcium sensor in hippocampal LTD

Palmer, Claire; Lim, Wonil; Hastie, Peter G R; Toward, Mark; Korolchuk, Victor I; Burbidge, Stephen A; Banting, George; Collingridge, Graham L; Isaac, John T R; Henley, Jeremy M

Abstract

It is not fully understood how NMDAR-dependent LTD causes Ca2+-dependent endocytosis of AMPARs. Here we show that the neuronal Ca2+ sensor hippocalcin binds the β2-adaptin subunit of the AP2 adaptor complex and that along with GluR2 these coimmunoprecipitate in a Ca2+-sensitive manner. Infusion of a truncated mutant of hippocalcin (HIP2-72) that lacks the Ca2+ binding domains prevents synaptically evoked LTD but has no effect on LTP. These data indicate that the AP2-hippocalcin complex acts as a Ca2+ sensor that couples NMDAR-dependent activation to regulated endocytosis of AMPARs during LTD.

• Journal Article Type: Article
• Publication Date: 2005-08
• Deposit Date: Jun 24, 2008
• Print ISSN: 0896-6273
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 47
• Issue: 4
• Pages: 487-494
• DOI: https://doi.org/10.1016/j.neuron.2005.06.014
• Keywords: AMPARs; Hippocalcin; NMDAR-dependent LTDs
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/1655
• Publisher URL: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSS-4GWS8KH-6&_user=132448&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000011019&_version=1&_urlVersion=0&_userid=132448&md5=49c4d0465373ec20c6a74c56ccc680c4