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Copper Homeostasis in Salmonella is atypical and Copper-CueP Is a major periplasmic metal complex

Osman, Deenah; Waldron, Kevin J; Denton, Harriet; Taylor, Clare M; Grant, Andrew J; Mastroeni, Pietro; Robinson, Nigel J; Cavet, Jennifer S

Authors

Deenah Osman

Kevin J Waldron

Harriet Denton

Andrew J Grant

Pietro Mastroeni

Nigel J Robinson

Jennifer S Cavet



Abstract

Salmonella enterica sv. typhimurium (S. enterica sv. Typhimurium) has two metal-transporting P1-type ATPases whose actions largely overlap with respect to growth in elevated copper. Mutants lacking both ATPases over-accumulate copper relative to wild-type or either single mutant. Such duplication of ATPases is unusual in bacterial copper tolerance. Both ATPases are under the control of MerR family metal-responsive transcriptional activators. Analyses of periplasmic copper complexes identified copper-CueP as one of the predominant metal pools. Expression of cueP was recently shown to be controlled by the same metal-responsive activator as one of the P1-type ATPase genes (copA), and copper-CueP is a further atypical feature of copper homeostasis in S. enterica sv. Typhimurium. Elevated copper is detected by a reporter construct driven by the promoter of copA in wild-type S. enterica sv. Typhimurium during infection of macrophages. Double mutants missing both ATPases also show reduced survival inside cultured macrophages. It is hypothesized that elevated copper within macrophages may have selected for specialized copper-resistance systems in pathogenic microorganism such as S. enterica sv. Typhimurium.

Citation

Osman, D., Waldron, K. J., Denton, H., Taylor, C. M., Grant, A. J., Mastroeni, P., …Cavet, J. S. (2010). Copper Homeostasis in Salmonella is atypical and Copper-CueP Is a major periplasmic metal complex. Journal of Biological Chemistry, 285, 25259-25268. https://doi.org/10.1074/jbc.M110.145953

Journal Article Type Article
Publication Date 2010
Deposit Date Jun 26, 2012
Print ISSN 0021-9258
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 285
Pages 25259-25268
DOI https://doi.org/10.1074/jbc.M110.145953
Keywords Salmonella enterica sv. typhimurium (S. enterica sv. Typhimurium); bacterial copper tolerance; copper-CueP;
Public URL http://researchrepository.napier.ac.uk/id/eprint/5511
Publisher URL http://dx.doi.org/10.1074/jbc.M110.145953



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