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Soybean ferritin expression in saccharomyces cerevisiae modulates iron accumulation and resistance to elevated iron concentrations.

de Llanos, Rosa; Mart�nez-Garay, Carlos Andr�s; Fita-Torr�, Josep; Romero, Antonia Mar�a; Mart�nez-Pastor, Mar�a Teresa; Puig, Sergi

Authors

Rosa de Llanos

Carlos Andr�s Mart�nez-Garay

Josep Fita-Torr�

Antonia Mar�a Romero

Mar�a Teresa Mart�nez-Pastor

Sergi Puig



Contributors

D. Cullen
Editor

Abstract

Fungi, including the yeast Saccharomyces cerevisiae, lack ferritin and use vacuoles as iron storage organelles. This work explored how plant ferritin expression influenced baker's yeast iron metabolism. Soybean seed ferritin H1 (SFerH1) and SFerH2 genes were cloned and expressed in yeast cells. Both soybean ferritins assembled as multimeric complexes, which bound yeast intracellular iron in vivo and, consequently, induced the activation of the genes expressed during iron scarcity. Soybean ferritin protected yeast cells that lacked the Ccc1 vacuolar iron detoxification transporter from toxic iron levels by reducing cellular oxidation, thus allowing growth at high iron concentrations. Interestingly, when simultaneously expressed in ccc1Δ cells, SFerH1 and SFerH2 assembled as heteropolymers, which further increased iron resistance and reduced the oxidative stress produced by excess iron compared to ferritin homopolymer complexes. Finally, soybean ferritin expression led to increased iron accumulation in both wild-type and ccc1Δ yeast cells at certain environmental iron concentrations.

Journal Article Type Article
Acceptance Date Mar 8, 2016
Online Publication Date Mar 11, 2016
Publication Date May 15, 2016
Deposit Date Jun 10, 2016
Journal Applied and Environmental Microbiology
Print ISSN 0099-2240
Electronic ISSN 1098-5336
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 82
Issue 10
Pages 3052-3060
DOI https://doi.org/10.1128/AEM.00305-16
Keywords Soybean; ferritin; expression; iron deficiency; nutritional disorder;
Public URL http://researchrepository.napier.ac.uk/id/eprint/10359
Publisher URL http://dx.doi.org/10.1128/AEM.00305-16