Soybean ferritin expression in saccharomyces cerevisiae modulates iron accumulation and resistance to elevated iron concentrations.

de Llanos, Rosa; Mart�nez-Garay, Carlos Andr�s; Fita-Torr�, Josep; Romero, Antonia Mar�a; Mart�nez-Pastor, Mar�a Teresa; Puig, Sergi

Abstract

Fungi, including the yeast Saccharomyces cerevisiae, lack ferritin and use vacuoles as iron storage organelles. This work explored how plant ferritin expression influenced baker's yeast iron metabolism. Soybean seed ferritin H1 (SFerH1) and SFerH2 genes were cloned and expressed in yeast cells. Both soybean ferritins assembled as multimeric complexes, which bound yeast intracellular iron in vivo and, consequently, induced the activation of the genes expressed during iron scarcity. Soybean ferritin protected yeast cells that lacked the Ccc1 vacuolar iron detoxification transporter from toxic iron levels by reducing cellular oxidation, thus allowing growth at high iron concentrations. Interestingly, when simultaneously expressed in ccc1Δ cells, SFerH1 and SFerH2 assembled as heteropolymers, which further increased iron resistance and reduced the oxidative stress produced by excess iron compared to ferritin homopolymer complexes. Finally, soybean ferritin expression led to increased iron accumulation in both wild-type and ccc1Δ yeast cells at certain environmental iron concentrations.

Citation

de Llanos, R., Martínez-Garay, C. A., Fita-Torró, J., Romero, A. M., Martínez-Pastor, M. T., & Puig, S. (2016). Soybean ferritin expression in saccharomyces cerevisiae modulates iron accumulation and resistance to elevated iron concentrations. Applied and Environmental Microbiology, 82(10), 3052-3060. https://doi.org/10.1128/AEM.00305-16

• Journal Article Type: Article
• Acceptance Date: Mar 8, 2016
• Online Publication Date: Mar 11, 2016
• Publication Date: May 15, 2016
• Deposit Date: Jun 10, 2016
• Journal: Applied and Environmental Microbiology
• Print ISSN: 0099-2240
• Electronic ISSN: 1098-5336
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 82
• Issue: 10
• Pages: 3052-3060
• DOI: https://doi.org/10.1128/AEM.00305-16
• Keywords: Soybean; ferritin; expression; iron deficiency; nutritional disorder
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/10359
• Publisher URL: http://dx.doi.org/10.1128/AEM.00305-16