PP2ACdc55’s role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function

Kerr, Gary W.; Wong, Jin Huei; Arumugam, Prakash

Abstract

PP2ACdc55 is a highly conserved serine-threonine protein phosphatase that is involved in diverse cellular processes. In budding yeast, meiotic cells lacking PP2ACdc55 activity undergo a premature exit from meiosis I which results in a failure to form bipolar spindles and divide nuclei. This defect is largely due to its role in negatively regulating the Cdc Fourteen Early Anaphase Release (FEAR) pathway. PP2ACdc55 prevents nucleolar release of the Cdk (Cyclin-dependent kinase)-antagonising phosphatase Cdc14 by counteracting phosphorylation of the nucleolar protein Net1 by Cdk. CDC55 was identified in a genetic screen for monopolins performed by isolating suppressors of spo11Δ spo12Δ lethality suggesting that Cdc55 might have a role in meiotic chromosome segregation. We investigated this possibility by isolating cdc55 alleles that suppress spo11Δ spo12Δ lethality and show that this suppression is independent of PP2ACdc55’s FEAR function. Although the suppressor mutations in cdc55 affect reductional chromosome segregation in the absence of recombination, they have no effect on chromosome segregation during wild type meiosis. We suggest that Cdc55 is required for reductional chromosome segregation during achiasmate meiosis and this is independent of its FEAR function.

Citation

Kerr, G. W., Wong, J. H., & Arumugam, P. (2016). PP2ACdc55’s role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function. Scientific Reports, 6(1), Article 30397. https://doi.org/10.1038/srep30397

• Journal Article Type: Article
• Acceptance Date: Jun 30, 2016
• Online Publication Date: Jul 26, 2016
• Publication Date: 2016-09
• Deposit Date: Feb 19, 2019
• Publicly Available Date: Feb 19, 2019
• Journal: Scientific Reports
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 1
• Article Number: 30397
• DOI: https://doi.org/10.1038/srep30397
• Keywords: serine-threonine protein phosphatase, cellular processes, Cdc55, reductional chromosome segregation,achiasmate meiosis,
• Public URL: http://researchrepository.napier.ac.uk/Output/1596274
• Publisher URL: https://www.nature.com/articles/srep30397
• Contract Date: Feb 19, 2019