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Meiotic nuclear divisions in budding yeast require PP2ACdc55-mediated antagonism of Net1 phosphorylation by Cdk

Kerr, Gary; Sarkar, Sourav; Tibbles, Katherine L.; Petronczki, Mark; Millar, Jonathan B.A.; Arumugam, Prakash

Authors

Sourav Sarkar

Katherine L. Tibbles

Mark Petronczki

Jonathan B.A. Millar

Prakash Arumugam



Abstract

During meiosis, one round of deoxyribonucleic acid replication is followed by two rounds of nuclear division. In Saccharomyces cerevisiae, activation of the Cdc14 early anaphase release (FEAR) network is required for exit from meiosis I but does not lead to the activation of origins of replication. The precise mechanism of how FEAR regulates meiosis is not understood. In this paper, we report that premature activation of FEAR during meiosis caused by loss of protein phosphatase PP2ACdc55 activity blocks bipolar spindle assembly and nuclear divisions. In cdc55 meiotic null (cdc55-mn) cells, the cyclin-dependent kinase (Cdk)–counteracting phosphatase Cdc14 was released prematurely from the nucleolus concomitant with hyperphosphorylation of its nucleolar anchor protein Net1. Crucially, a mutant form of Net1 that lacks six Cdk phosphorylation sites rescued the meiotic defect of cdc55-mn cells. Expression of a dominant mutant allele of CDC14 mimicked the cdc55-mn phenotype. We propose that phosphoregulation of Net1 by PP2ACdc55 is essential for preventing precocious exit from meiosis I.

Journal Article Type Article
Acceptance Date May 25, 2011
Online Publication Date Jun 20, 2011
Publication Date Jun 27, 2011
Deposit Date Feb 19, 2019
Publicly Available Date Mar 5, 2019
Journal Journal of Cell Biology
Print ISSN 0021-9525
Publisher Rockefeller University Press
Peer Reviewed Peer Reviewed
Volume 193
Issue 7
Pages 1157-1166
Public URL http://researchrepository.napier.ac.uk/Output/1596241
Contract Date Mar 5, 2019

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http://creativecommons.org/licenses/by-nc/3.0/

Copyright Statement
© 2011 Kerr et al. This article is distributed under the terms of an Attribution–
Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).







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