Skip to main content

Research Repository

Advanced Search

Analysing the effect of dietary prebiotics and probiotics on gut bacterial richness and diversity of Asian snakehead fingerlings using T-RFLP method

Munir, Mohammad Bodrul; Marsh, Terence L.; Blaud, Aimeric; Hashim, Roshada; Janti Anak Joshua, Wizilla; Mohd Nor, Siti Azizah

Authors

Mohammad Bodrul Munir

Terence L. Marsh

Roshada Hashim

Wizilla Janti Anak Joshua

Siti Azizah Mohd Nor



Abstract

This study was designed to evaluate the effects of three prebiotics (β‐glucan, galacto‐oligosaccharide [GOS], mannan‐oligosaccharide [MOS]) and two probiotics (Saccharomyces cerevisiae, Lactobacillus acidophilus) on the microbiome of snakehead during growth of fingerlings. In addition, the experiment evaluated the capacity of Channa striata fingerlings to retain the benefits derived from these supplements after withdrawal. Throughout the study, it was observed that supplementation with dietary prebiotics and probiotics led to significant (p < 0.05) change in gut bacterial profile and improvement in gut morphology. Terminal restriction fragment length polymorphism (T‐RFLP) was used for the comparative analysis of gut communities and all 46 of the T‐RFLP detected phylotypes were present in the Lactobacillus supplemented fish, while significantly fewer were detected in controls and other experimental supplement regimes. Histological studies and electron microscopy revealed that both the prebiotic and probiotic treated fish had significantly longer and wider villi and deeper crypts compared to the controls. The microvilli length, as evaluated with electron microscopy, was also longer in all treated fish compared to controls. Furthermore, this study is the first to report the absence of differences in sustaining the efficacies attained after intake of β‐glucan, GOS, MOS and live yeast upon post‐feeding with an unsupplemented feed, over a prolonged period.

Journal Article Type Article
Acceptance Date Jul 14, 2018
Online Publication Date Aug 15, 2018
Publication Date 2018-10
Deposit Date Nov 30, 2018
Journal Aquaculture Research
Print ISSN 1355-557X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 49
Issue 10
Pages 3350-3361
DOI https://doi.org/10.1111/are.13799
Keywords gut bacterial richness and diversity, prebiotics, probiotics, snakehead fingerlings, terminal restriction, fragment length, polymorphism
Public URL http://researchrepository.napier.ac.uk/Output/1348483
Publisher URL https://onlinelibrary.wiley.com/doi/pdf/10.1111/are.13799