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Intrafamilial genotyping of helicobacter pylori from faecal DNA

McMillan, M.; MacKay, W. G.; Williams, C. L.; Shepherd, A. J.; Malcolm, C.; Weaver, L. T.


M. McMillan

W. G. MacKay

C. L. Williams

A. J. Shepherd

C. Malcolm

L. T. Weaver


Helicobacter pylori infection, often acquired in early childhood, is a global cause of undernutrition, gastritis, peptic ulcer disease and gastric carcinoma. This study tested the feasibility of using H. pylori shed in the faeces as a source of DNA for non-invasive epidemiological studies. H. pylori DNA was chemically recovered and isolated using a specific biotinylated oligonucleotide probe with magnetic capture from 28 H. pylori positive faecal samples obtained from children attending hospital for the investigation of suspected H. pylori infection, together with close family members. Random amplification of polymorphic DNA (RAPD) was subsequently used to discriminate each isolate. 93% of stool samples selected were typeable. Parent, child and sibling samples were compared and similarities determined. Phylogenetic analysis showed that H. pylori DNA obtained from the faeces can be used to genotype individual strains, offering a means of studying intrafamilial transfer of this microorganism.


McMillan, M., MacKay, W. G., Williams, C. L., Shepherd, A. J., Malcolm, C., & Weaver, L. T. (2011). Intrafamilial genotyping of helicobacter pylori from faecal DNA. Gastroenterology Research and Practice, 2011,

Journal Article Type Article
Acceptance Date May 23, 2011
Online Publication Date Jul 1, 2011
Publication Date 2011
Deposit Date Jul 27, 2017
Publicly Available Date Jul 27, 2017
Journal Gastroenterology Research and Practice
Print ISSN 1687-6121
Electronic ISSN 1687-630X
Publisher Hindawi
Peer Reviewed Peer Reviewed
Volume 2011
Article Number 491035
Keywords Hepatology; Gastroenterology,
Public URL


Intrafamilial genotyping of helicobacter pylori from faecal DNA. (<nobr>2.2 Mb</nobr>)

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