Jérémy Puissant
The pH optimum of soil exoenzymes adapt to long term changes in soil pH
Puissant, Jérémy; Jones, Briony; Goodall, Tim; Mang, Dana; Blaud, Aimeric; Gweon, Hyun Soon; Malik, Ashish; Jones, Davey L.; Clark, Ian M.; Hirsch, Penny R.; Griffiths, Robert
Authors
Briony Jones
Tim Goodall
Dana Mang
Dr Aimeric Blaud A.Blaud@napier.ac.uk
Lecturer
Hyun Soon Gweon
Ashish Malik
Davey L. Jones
Ian M. Clark
Penny R. Hirsch
Robert Griffiths
Abstract
Soil exoenzymes released by microorganisms break down organic matter and are crucial in regulating C, N and P cycling. Soil pH is known to influence enzyme activity, and is also a strong driver of microbial community composition; but little is known about how alterations in soil pH affect enzymatic activity and how this is mediated by microbial communities. To assess long term enzymatic adaptation to soil pH, we conducted enzyme assays at buffered pH levels on two historically managed soils maintained at either pH 5 or 7 from the Rothamsted Park Grass Long-term experiment. The pH optima for a range of exoenzymes involved in C, N, P cycling, differed between the two soils, the direction of the shift being toward the source soil pH, indicating the production of pH adapted isoenzymes by the soil microbial community. Soil bacterial and fungal communities determined by amplicon sequencing were clearly distinct between pH 5 and soil pH 7 soils, possibly explaining differences in enzymatic responses. Furthermore, β-glucosidase gene sequences extracted from metagenomes revealed an increased abundance of Acidobacterial producers in the pH 5 soils, and Actinobacteria in pH 7 soils. Our findings demonstrate that the pH optimum of soil exoenzymes adapt to long term changes in soil pH, the direction being dependent on the soil pH shift; and we provide further evidence that changes in functional microbial communities may underpin this phenomena, though new research is now needed to directly link change in enzyme activity optima with microbial communities. More generally, our new findings have large implications for modelling the efficiency of different microbial enzymatic processes under changing environmental conditions.
Citation
Puissant, J., Jones, B., Goodall, T., Mang, D., Blaud, A., Gweon, H. S., …Griffiths, R. (2019). The pH optimum of soil exoenzymes adapt to long term changes in soil pH. Soil Biology and Biochemistry, 138, 107601. https://doi.org/10.1016/j.soilbio.2019.107601
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 15, 2019 |
Online Publication Date | Sep 16, 2019 |
Publication Date | 2019-11 |
Deposit Date | Oct 3, 2019 |
Publicly Available Date | Sep 17, 2020 |
Journal | Soil Biology and Biochemistry |
Print ISSN | 0038-0717 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 138 |
Pages | 107601 |
DOI | https://doi.org/10.1016/j.soilbio.2019.107601 |
Keywords | Enzyme activity, Adaptation, Liming, Carbon degradation, Metagenomics, Microbial community |
Public URL | http://researchrepository.napier.ac.uk/Output/2150661 |
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The PH Optimum Of Soil Exoenzymes Adapt To Long Term Changes In Soil PH (accepted manuscript)
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Publisher Licence URL
http://creativecommons.org/licenses/by-nc-nd/4.0/
Copyright Statement
This accepted manuscript is released with a Creative Commons Attribution Non-Commercial No Derivatives License (CC BY-NC-ND).
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