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Microbes associated with fresh produce: sources, types and methods to reduce spoilage and contamination (2019)
Book Chapter
Kaczmarek, M., Avery, S. V., & Singleton, I. (2019). Microbes associated with fresh produce: sources, types and methods to reduce spoilage and contamination. In G. M. Gadd, & S. Sariaslani (Eds.), Advances in Applied Microbiology. Vol 107 (29-82). Elsevier. https://doi.org/10.1016/bs.aambs.2019.02.001

Global food security remains one of the most important challenges that needs to be addressed to ensure the increasing demand for food of the fast growing human population is satisfied. Fruits and vegetables comprise an essential component of a health... Read More about Microbes associated with fresh produce: sources, types and methods to reduce spoilage and contamination.

Sources and survival of Listeria monocytogenes on fresh, leafy produce (2018)
Journal Article
on fresh, leafy produce. Journal of Applied Microbiology, 125(4), 930-942. https://doi.org/10.1111/jam.14025

Listeria monocytogenes in an intracellular human pathogen which enters the body through contaminated food stuffs and is known to contaminate fresh leafy produce such as spinach, lettuce and rocket. Routinely, fresh leafy produce is grown and processe... Read More about Sources and survival of Listeria monocytogenes on fresh, leafy produce.

Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review (2018)
Journal Article
Alegbeleye, O. O., Singleton, I., & Sant’Ana, A. S. (2018). Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review. Food Microbiology, 73, 177-208. https://doi.org/10.1016/j.fm.2018.01.003

Foodborne illness resulting from the consumption of contaminated fresh produce is a common phenomenon and has severe effects on human health together with severe economic and social impacts. The implications of foodborne diseases associated with fres... Read More about Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review.

Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera (2017)
Journal Article
Sweet, M. J., Brown, B. E., Dunne, R. P., Singleton, I., & Bulling, M. (2017). Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera. Coral Reefs, 36(3), 815-828. https://doi.org/10.1007/s00338-017-1572-y

Shifts in the microbiome of the intertidal coral Coelastrea aspera (formally known as Goniastrea aspera) from Phuket, Thailand were noted over the course of a four day period of spring tides. During this time corals were naturally exposed to high tem... Read More about Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera.

Isolation of Indigenous Hydrocarbon Transforming Bacteria from Oil Contaminated Soils in Libya: Selection for Use as Potential Inocula for Soil Bioremediation (2017)
Journal Article
Hakima, A., & Singleton, I. (2017). Isolation of Indigenous Hydrocarbon Transforming Bacteria from Oil Contaminated Soils in Libya: Selection for Use as Potential Inocula for Soil Bioremediation. International journal of environmental bioremediation & biodegradation, 5(1), 8-17. https://doi.org/10.12691/ijebb-5-1-2

The Libyan oil industry has left a significant legacy of contamination and methods are required to remediate oil-contaminated soils in the area. In this work hydrocarbon utilizing microorganisms were isolated and identified from contaminated soil sam... Read More about Isolation of Indigenous Hydrocarbon Transforming Bacteria from Oil Contaminated Soils in Libya: Selection for Use as Potential Inocula for Soil Bioremediation.

Chapter Five - Microsatellites for Microbiologists (2012)
Book
Sweet, M. J., Scriven, L. A., & Singleton, I. (2012). Chapter Five - Microsatellites for Microbiologists. Elsevier. https://doi.org/10.1016/B978-0-12-394382-8.00005-8

Abstract Microsatellites are repeating sequences of 2–6 base pairs of DNA. Currently, they are used as molecular markers in many organisms, specifically in genetic studies analyzing kinship and population structure. In addition, they can be used to s... Read More about Chapter Five - Microsatellites for Microbiologists.

Effect of ozone on spore germination, spore production and biomass production in two Aspergillus species (2009)
Journal Article
Antony-Babu, S., & Singleton, I. (2009). Effect of ozone on spore germination, spore production and biomass production in two Aspergillus species. Antonie van Leeuwenhoek, 96(4), 413-422. https://doi.org/10.1007/s10482-009-9355-2

The ability of ozone gas to reduce food spoilage is relatively well documented, but the developmental effects of the gas on food spoilage fungi are not well known. In this study two model aspergilli, Aspergillus nidulans and Aspergillus ochraceus wer... Read More about Effect of ozone on spore germination, spore production and biomass production in two Aspergillus species.

Biological tools to assess contaminant bioavailability in soils (2008)
Book Chapter
Keatinge, Z., & Singleton, I. (2008). Biological tools to assess contaminant bioavailability in soils. In Chemical Bioavailability in Terrestrial Environment; Developments in Soil Science (479-494). Elsevier. https://doi.org/10.1016/s0166-2481%2807%2932019-9

This chapter provides an overview of the biological tools to assess contaminants bioavailability in soils. Compounds that cause contamination include heavy metals, polyaromatic hydrocarbons (PAHs), pesticides, and chlorinated solvents. For the purpos... Read More about Biological tools to assess contaminant bioavailability in soils.

Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit (2007)
Journal Article
Tzortzakis, N., Singleton, I., & Barnes, J. (2008). Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit. Postharvest Biology and Technology, 47(1), 1-9. https://doi.org/10.1016/j.postharvbio.2007.06.004

Tomato fruit (Lycopersicon esculentum L.) were exposed to ozone concentrations between 0.005 (control) and 5.0 μmol mol−1 up to 13 days at 13 °C, prior to, or following, inoculation by Alternaria alternata or Colletotrichum coccodes (causes of black... Read More about Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit.

Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit (2007)
Journal Article
Tzortzakis, N., Borland, A., Singleton, I., & Barnes, J. (2007). Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit. Postharvest Biology and Technology, 45(3), 317-325. https://doi.org/10.1016/j.postharvbio.2007.03.004

Tomato fruit (Lycopersicon esculentum L. cv. Carousel) were exposed to ozone concentrations ranging between 0.005 (controls) and 1.0 μmol mol−1 at 13 °C and 95% RH. Quality-related attributes and organoleptic characteristics were examined during and... Read More about Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit.

Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce (2007)
Journal Article
Tzortzakis, N., Singleton, I., & Barnes, J. (2007). Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce. Postharvest Biology and Technology, 43(2), 261-270. https://doi.org/10.1016/j.postharvbio.2006.09.005

Tomatoes, strawberries, table grapes and plums were inoculated with Botrytis cinerea (grey mould), transferred to chilled storage (13 °C) and exposed to ‘clean air’ or low-level ozone-enrichment (0.1 μmol mol−1). Ozone-enrichment resulted in a substa... Read More about Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce.

Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS) (2007)
Journal Article
Hua, G., Broderick, J., Semple, K. T., Killham, K., & Singleton, I. (2007). Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS). Environmental Pollution, 148(1), 176-181. https://doi.org/10.1016/j.envpol.2006.10.040

Synchronous fluorescence spectroscopy (SFS) was directly applied to rapidly quantify selected polycyclic aromatic hydrocarbons (PAHs: benzo[a]pyrene and pyrene) in aqueous hydroxypropyl-β-cyclodextrin (HPCD) soil extract solutions from a variety of a... Read More about Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS).

Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting (2006)
Journal Article
Vizard, C. G., Rimmer, D. L., Pless-Mulloli, T., Singleton, I., & Air, V. S. (2006). Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting. Science of the Total Environment, 370(1), 61-69. https://doi.org/10.1016/j.scitotenv.2006.06.006

A study of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/F) concentrations was undertaken in the vicinity of a municipal solid waste incinerator (MSWI) in Newcastle upon Tyne as a result of concerns raised by local re... Read More about Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting.

Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. (2006)
Journal Article
Lee, M., Kim, M., Singleton, I., Goodfellow, M., & Lee, S.-T. (2006). Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. Journal of Applied Microbiology, 100(2), 325-333. https://doi.org/10.1111/j.1365-2672.2005.02756.x

Aims:  The aim of the present study was to isolate and characterize a bacterium, strain EN3, capable of using diesel oil as a major carbon and energy source, and to analyse the enhancement of diesel oil degradation by this organism using synthetic my... Read More about Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid..

Environmental Pollution and Restoration: A Role for Bioremediation (2005)
Book Chapter
Philp, J. C., Bamforth, S., Singleton, I., & Atlas, R. M. (2005). Environmental Pollution and Restoration: A Role for Bioremediation. In R. M. Atlas, & J. Philp (Eds.), Bioremediation (1-48). American Society for Microbiology. https://doi.org/10.1128/9781555817596.ch1

With greater understanding of microbial diversity and the development of bioengineering, bioremediation is taking its place as a cost-effective technique in integrated environmental restoration efforts. The major reasons for the control of water and... Read More about Environmental Pollution and Restoration: A Role for Bioremediation.

Fungal remediation of soils contaminated with persistent organic pollutants (2001)
Book Chapter
Singleton, I. (2001). Fungal remediation of soils contaminated with persistent organic pollutants. In G. M. Gadd (Ed.), Fungi in Bioremediation (79-96). Cambridge University Press. https://doi.org/10.1017/CBO9780511541780.005

Laboratory-based studies have shown that fungi are able to degrade a wide range of organic pollutants (see other chapters) and have great potential for use as inoculants to remediate contaminated soil. However, soil is a heterogeneous environment and... Read More about Fungal remediation of soils contaminated with persistent organic pollutants.

Pentachlorophenol transformation in soil: a toxicological assessment (2000)
Journal Article
McGrath, R., & Singleton, I. (2000). Pentachlorophenol transformation in soil: a toxicological assessment. Soil Biology and Biochemistry, 32(8-9), 1311-1314. https://doi.org/10.1016/s0038-0717%2800%2900015-8

Bioremediation is recognised as an economically viable method to treat contaminated soil and the use of microbial inoculants, in particular, white-rot fungi, has been proposed to enhance the remediation process. During bioremediation, a variety of po... Read More about Pentachlorophenol transformation in soil: a toxicological assessment.

Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil (2000)
Journal Article
Megharaj, M., Singleton, I., McClure, N. C., & Naidu, R. (2000). Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil. Archives of Environmental Contamination and Toxicology, 38(4), 439-445. https://doi.org/10.1007/s002449910058

Petroleum hydrocarbons are widespread environmental pollutants. Although biodegradation of petroleum hydrocarbons has been the subject of numerous investigations, information on their toxicity to microorganisms in soil is limited, with virtually no w... Read More about Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil.

Effects of long-term contamination of DDT on soil microflora with special reference to soil algae and algal transformation of DDT (2000)
Journal Article
Megharaj, M., Kantachote, D., Singleton, I., & Naidu, R. (2000). Effects of long-term contamination of DDT on soil microflora with special reference to soil algae and algal transformation of DDT. Environmental Pollution, 109(1), 35-42. https://doi.org/10.1016/s0269-7491%2899%2900231-6

DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane) and its principle metabolites, DDE (1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene) and DDD (1,1-dichloro-2,2-bis(p-chlorophenyl)ethane) are widespread environmental contaminants but little informatio... Read More about Effects of long-term contamination of DDT on soil microflora with special reference to soil algae and algal transformation of DDT.