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Microbes associated with fresh produce: sources, types and methods to reduce spoilage and contamination (2019)
Book Chapter
Kaczmarek, M., Avery, S. V., & Singleton, I. (2019). Microbes associated with fresh produce: sources, types and methods to reduce spoilage and contamination. In G. M. Gadd, & S. Sariaslani (Eds.), Advances in Applied Microbiology. Vol 107 (29-82). Elsevier. https://doi.org/10.1016/bs.aambs.2019.02.001

Global food security remains one of the most important challenges that needs to be addressed to ensure the increasing demand for food of the fast growing human population is satisfied. Fruits and vegetables comprise an essential component of a health... Read More about Microbes associated with fresh produce: sources, types and methods to reduce spoilage and contamination.

Sources and survival of Listeria monocytogenes on fresh, leafy produce (2018)
Journal Article
on fresh, leafy produce. Journal of Applied Microbiology, 125(4), 930-942. https://doi.org/10.1111/jam.14025

Listeria monocytogenes in an intracellular human pathogen which enters the body through contaminated food stuffs and is known to contaminate fresh leafy produce such as spinach, lettuce and rocket. Routinely, fresh leafy produce is grown and processe... Read More about Sources and survival of Listeria monocytogenes on fresh, leafy produce.

Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review (2018)
Journal Article
Alegbeleye, O. O., Singleton, I., & Sant’Ana, A. S. (2018). Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review. Food Microbiology, 73, 177-208. https://doi.org/10.1016/j.fm.2018.01.003

Foodborne illness resulting from the consumption of contaminated fresh produce is a common phenomenon and has severe effects on human health together with severe economic and social impacts. The implications of foodborne diseases associated with fres... Read More about Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review.

Silver Nanoparticles: A Microbial Perspective (2011)
Book Chapter
Sweet, M., & Singleton, I. (2011). Silver Nanoparticles: A Microbial Perspective. . Academic Press. https://doi.org/10.1016/B978-0-12-387044-5.00005-4

Abstract Silver nanoparticles (NPs) are used for a wide range of commercial reasons to restrict microbial growth. The increasing use of silver {NPs} in modern materials ensures they will find their way into environmental systems. The mode of action w... Read More about Silver Nanoparticles: A Microbial Perspective.

Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomato fruit (2011)
Journal Article
Tzortzakis, N., Taybi, T., Roberts, R., Singleton, I., Borland, A., & Barnes, J. (2011). Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomato fruit. Postharvest Biology and Technology, 61(2-3), 152-159. https://doi.org/10.1016/j.postharvbio.2011.02.013

The aim of this study was to determine if ozone exposure could prevent spoilage in tomato fruit by fungal infection and to explore concomitant changes in expression of genes involved in signal transduction (ethylene, jasmonic acid and C6-aldehydes) a... Read More about Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomato fruit.

Quinol-cytochrome c Oxidoreductase and Cytochrome c4 Mediate Electron Transfer during Selenate Respiration in Thauera selenatis (2010)
Journal Article
Lowe, E. C., Bydder, S., Hartshorne, R. S., Tape, H. L. U., Dridge, E. J., Debieux, C. M., Paszkiewicz, K., Singleton, I., Lewis, R. J., Santini, J. M., Richardson, D. J., & Butler, C. S. (2010). Quinol-cytochrome c Oxidoreductase and Cytochrome c4 Mediate Electron Transfer during Selenate Respiration in Thauera selenatis. Journal of Biological Chemistry, 285(24), 18433-18442. https://doi.org/10.1074/jbc.M110.115873

Selenate reductase (SER) from Thauera selenatis is a periplasmic enzyme that has been classified as a type II molybdoenzyme. The enzyme comprises three subunits SerABC, where SerC is an unusual b-heme cytochrome. In the present work the spectropotent... Read More about Quinol-cytochrome c Oxidoreductase and Cytochrome c4 Mediate Electron Transfer during Selenate Respiration in Thauera selenatis.

Potential use of DNA adducts to detect mutagenic compounds in soil (2008)
Journal Article
Hua, G., Lyons, B., Killham, K., & Singleton, I. (2009). Potential use of DNA adducts to detect mutagenic compounds in soil. Environmental Pollution, 157(3), 916-921. https://doi.org/10.1016/j.envpol.2008.10.026

In this study, three different soils with contrasting features, spiked with 300 mg benzo[a]pyrene (BaP)/kg dry soil, were incubated at 20 °C and 60% water holding capacity for 540 days. At different time points, BaP and {DNA} were extracted and quant... Read More about Potential use of DNA adducts to detect mutagenic compounds in soil.

Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting (2006)
Journal Article
Vizard, C. G., Rimmer, D. L., Pless-Mulloli, T., Singleton, I., & Air, V. S. (2006). Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting. Science of the Total Environment, 370(1), 61-69. https://doi.org/10.1016/j.scitotenv.2006.06.006

A study of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/F) concentrations was undertaken in the vicinity of a municipal solid waste incinerator (MSWI) in Newcastle upon Tyne as a result of concerns raised by local re... Read More about Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting.

Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. (2006)
Journal Article
Lee, M., Kim, M., Singleton, I., Goodfellow, M., & Lee, S.-T. (2006). Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. Journal of Applied Microbiology, 100(2), 325-333. https://doi.org/10.1111/j.1365-2672.2005.02756.x

Aims:  The aim of the present study was to isolate and characterize a bacterium, strain EN3, capable of using diesel oil as a major carbon and energy source, and to analyse the enhancement of diesel oil degradation by this organism using synthetic my... Read More about Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid..

Fungal remediation of soils contaminated with persistent organic pollutants (2001)
Book Chapter
Singleton, I. (2001). Fungal remediation of soils contaminated with persistent organic pollutants. In G. M. Gadd (Ed.), Fungi in Bioremediation (79-96). Cambridge University Press. https://doi.org/10.1017/CBO9780511541780.005

Laboratory-based studies have shown that fungi are able to degrade a wide range of organic pollutants (see other chapters) and have great potential for use as inoculants to remediate contaminated soil. However, soil is a heterogeneous environment and... Read More about Fungal remediation of soils contaminated with persistent organic pollutants.

Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil (2000)
Journal Article
Megharaj, M., Singleton, I., McClure, N. C., & Naidu, R. (2000). Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil. Archives of Environmental Contamination and Toxicology, 38(4), 439-445. https://doi.org/10.1007/s002449910058

Petroleum hydrocarbons are widespread environmental pollutants. Although biodegradation of petroleum hydrocarbons has been the subject of numerous investigations, information on their toxicity to microorganisms in soil is limited, with virtually no w... Read More about Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil.

Fungal interactions with metals and radionuclides for environmental bioremediation (1996)
Book Chapter
Singleton, I., & Tobin, J. M. (1996). Fungal interactions with metals and radionuclides for environmental bioremediation. In J. C. Frankland, N. Magan, & G. M. Gadd (Eds.), Fungi and environmental change (282-298). Cambridge University Press. https://doi.org/10.1017/CBO9780511753190.018

Microorganisms, including fungi, are known to accumulate metals from their external environment and the possibility of using fungi as a means of treating metal/radionuclide-containing effluents is well recognized (Siegel, Galun & Siegel, 1990; Gadd,... Read More about Fungal interactions with metals and radionuclides for environmental bioremediation.

Factors affecting silver biosorption by an industrial strain of Saccharomyces cerevisiae (1996)
Journal Article
Singleton, I., & Simmons, P. (1996). Factors affecting silver biosorption by an industrial strain of Saccharomyces cerevisiae. Journal of Chemical Technology and Biotechnology, 65(1), 21-28. https://doi.org/10.1002/%28SICI%291097-4660%28199601%2965%3A13.0.CO%3B2-E

Factors affecting silver biosorption by Saccharomyces cerevisiae biomass, obtained as a waste product from industry, were examined. Maximum removal of silver from solution was achieved within 5 min. Increasing the concentration of biomass in experime... Read More about Factors affecting silver biosorption by an industrial strain of Saccharomyces cerevisiae.

Metal cation uptake by yeast: a review (1995)
Journal Article
Blackwell, K. J., Singleton, I., & Tobin, J. M. (1995). Metal cation uptake by yeast: a review. Applied microbiology and biotechnology, 43(4), 579-584. https://doi.org/10.1007/s002530050454

This review addresses metal uptake specifically by yeast. Metal uptake may be passive, active or both, depending on the viability of the biomass, and is influenced by a number of environmental and experimental factors. Uptake is typically accompanied... Read More about Metal cation uptake by yeast: a review.

Microbial metabolism of xenobiotics: Fundamental and applied research (1994)
Journal Article
Singleton, I. (1994). Microbial metabolism of xenobiotics: Fundamental and applied research. Journal of Chemical Technology and Biotechnology, 59(1), 9-23. https://doi.org/10.1002/jctb.280590104

The ability of microorganisms to metabolise xenobiotic compounds has received much attention due to the environmental persistence and toxicity of these chemicals. The microbial degradation of xenobiotics is seen as a cost effective method of removing... Read More about Microbial metabolism of xenobiotics: Fundamental and applied research.