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All Outputs (63)

Pregnancy length and health in giant pandas: what can metabolic and urinary endocrine markers unveil? (2023)
Journal Article
Wauters, J., Wilson, K. S., Cools, T., Vancsok, C., Bouts, T., Mulot, B., …Li, D. (2023). Pregnancy length and health in giant pandas: what can metabolic and urinary endocrine markers unveil?. Theriogenology Wild, 3, Article 100063. https://doi.org/10.1016/j.therwi.2023.100063

Mature female giant pandas usually ovulate once a year. This is followed by an obligatory luteal phase, consisting of a long-lasting corpus luteum dormancy phase (CLD; primary increase in progestogens) and a much shorter active luteal phase (AL; seco... Read More about Pregnancy length and health in giant pandas: what can metabolic and urinary endocrine markers unveil?.

Development of an acute ovine model of polycystic ovaries to assess the effect of ovarian denervation (2023)
Journal Article
Duncan, W. C., Nicol, L. M., O’Hare, R., Witherington, J., Miranda, J. A., Campbell, B. K., …Rae, M. T. (2023). Development of an acute ovine model of polycystic ovaries to assess the effect of ovarian denervation. Frontiers in Endocrinology, 14, Article 1285269. https://doi.org/10.3389/fendo.2023.1285269

Introduction: Polycystic ovary syndrome (PCOS) seems to be associated with increased ovarian sympathetic nerve activity and in rodent models of PCOS reducing the sympathetic drive to the ovary, through denervation or neuromodulation, improves ovulati... Read More about Development of an acute ovine model of polycystic ovaries to assess the effect of ovarian denervation.

Hepatic Mitochondrial Dysfunction and Risk of Liver Disease in an Ovine Model of “PCOS Males” (2022)
Journal Article
Siemienowicz, K. J., Filis, P., Thomas, J., Fowler, P. A., Duncan, W. C., & Rae, M. T. (2022). Hepatic Mitochondrial Dysfunction and Risk of Liver Disease in an Ovine Model of “PCOS Males”. Biomedicines, 10(6), Article 1291. https://doi.org/10.3390/biomedicines10061291

First-degree male relatives of polycystic ovary syndrome (PCOS) sufferers can develop metabolic abnormalities evidenced by elevated circulating cholesterol and triglycerides, suggestive of a male PCOS equivalent. Similarly, male sheep overexposed to... Read More about Hepatic Mitochondrial Dysfunction and Risk of Liver Disease in an Ovine Model of “PCOS Males”.

Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells (2021)
Journal Article
Brown, M., Rae, M., & Wheelhouse, N. (2021). Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells. Reproduction and Fertility, 2(1), 9-11. https://doi.org/10.1530/RAF-20-0069

Chlamydia trachomatis is the most commonly diagnosed sexually transmitted infection in the UK, with over 200,000 positive diagnoses annually. The infection is thought to cause reproductive complications including problems in conceiving a pregnancy th... Read More about Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells.

Pubertal FGF21 deficit is central in the metabolic pathophysiology of an ovine model of polycystic ovary syndrome (2021)
Journal Article
Siemienowicz, K. J., Furmanska, K., Filis, P., Talia, C., Thomas, J., Fowler, P. A., …Colin Duncan, W. (2021). Pubertal FGF21 deficit is central in the metabolic pathophysiology of an ovine model of polycystic ovary syndrome. Molecular and Cellular Endocrinology, 525, Article 111196. https://doi.org/10.1016/j.mce.2021.111196

Polycystic ovary syndrome (PCOS), affecting over 10% of women, is associated with insulin resistance, obesity, dyslipidaemia, fatty liver and adipose tissue dysfunction. Its pathogenesis is poorly understood and consequently treatment remains subopti... Read More about Pubertal FGF21 deficit is central in the metabolic pathophysiology of an ovine model of polycystic ovary syndrome.

Early pregnancy maternal progesterone administration alters pituitary and testis function and steroid profile in male fetuses (2020)
Journal Article
Siemienowicz, K. J., Wang, Y., Marečková, M., Nio-Kobayashi, J., Fowler, P. A., Rae, M. T., & Duncan, W. C. (2020). Early pregnancy maternal progesterone administration alters pituitary and testis function and steroid profile in male fetuses. Scientific Reports, 10(1), https://doi.org/10.1038/s41598-020-78976-x

Maternal exposure to increased steroid hormones, including estrogens, androgens or glucocorticoids during pregnancy results in chronic conditions in offspring that manifest in adulthood. Little is known about effects of progesterone administration in... Read More about Early pregnancy maternal progesterone administration alters pituitary and testis function and steroid profile in male fetuses.

Aberrant subcutaneous adipogenesis precedes adult metabolic dysfunction in an ovine model of polycystic ovary syndrome (PCOS) (2020)
Journal Article
Siemienowicz, K. J., Coukan, F., Franks, S., Rae, M. T., & Colin Duncan, W. (2021). Aberrant subcutaneous adipogenesis precedes adult metabolic dysfunction in an ovine model of polycystic ovary syndrome (PCOS). Molecular and Cellular Endocrinology, 519, Article 111042. https://doi.org/10.1016/j.mce.2020.111042

Polycystic ovary syndrome (PCOS) affects over 10% of women. Insulin resistance, elevated free fatty acids (FFAs) and increased adiposity are key factors contributing to metabolic dysfunction in PCOS. We hypothesised that aberrant adipogenesis duri... Read More about Aberrant subcutaneous adipogenesis precedes adult metabolic dysfunction in an ovine model of polycystic ovary syndrome (PCOS).

Insights into manipulating postprandial energy expenditure to manage weight gain in polycystic ovary syndrome (PCOS) (2020)
Journal Article
Siemienowicz, K., Rae, M. T., Howells, F., Anderson, C., Nicol, L. M., Franks, S., & Duncan, W. C. (2020). Insights into manipulating postprandial energy expenditure to manage weight gain in polycystic ovary syndrome (PCOS). iScience, 23(6), https://doi.org/10.1016/j.isci.2020.101164

Women with polycystic ovary syndrome (PCOS) are more likely to be obese and have difficulty in losing weight. They demonstrate an obesity-independent deficit in adaptive energy expenditure. We used a clinically-realistic preclinical model to investig... Read More about Insights into manipulating postprandial energy expenditure to manage weight gain in polycystic ovary syndrome (PCOS).

Dyslipidaemia and altered hepatic function in males - consequences of androgen excess in fetal life (2019)
Journal Article
Siemienowicz, K., Filis, P., Shaw, S., Douglas, A., Thomas, J., Howie, F., …Rae, M. (2019). Dyslipidaemia and altered hepatic function in males - consequences of androgen excess in fetal life. Endocrine abstracts, 63, https://doi.org/10.1530/endoabs.63.P669

Introduction: Adult male offspring of women with PCOS have increased dyslipidaemia, characterised by elevated triglycerides (TG), increased total and LDL-cholesterol (LDL-C), and hyperinsulinaemia. As altered intrauterine endocrine environments can ‘... Read More about Dyslipidaemia and altered hepatic function in males - consequences of androgen excess in fetal life.

Fetal androgen exposure is a determinant of adult male metabolic health (2019)
Journal Article
Siemienowicz, K. J., Filis, P., Shaw, S., Douglas, A., Thomas, J., Mulroy, S., …Rae, M. T. (2019). Fetal androgen exposure is a determinant of adult male metabolic health. Scientific Reports, 9, Article 20195 (2019). https://doi.org/10.1038/s41598-019-56790-4

Androgen signalling is a critical driver of male development. Fetal steroid signalling can be dysregulated by a range of environmental insults and clinical conditions. We hypothesised that poor adult male health was partially attributable to aberrant... Read More about Fetal androgen exposure is a determinant of adult male metabolic health.

Urinary estrogens as a non-invasive biomarker of viable pregnancy in the giant panda (Ailuropoda melanoleuca) (2019)
Journal Article
Wilson, K. S., Wauters, J., Valentine, I., McNeilly, A., Girling, S., Li, R., …Duncan, W. C. (2019). Urinary estrogens as a non-invasive biomarker of viable pregnancy in the giant panda (Ailuropoda melanoleuca). Scientific Reports, 9(1), Article 12772. https://doi.org/10.1038/s41598-019-49288-6

Female giant pandas show complex reproductive traits, being seasonally monoestrus, displaying a variable length embryonic diapause and exhibiting pseudopregnancy. Currently, there is no confirmatory non-invasive biomarker of blastocyst implantation o... Read More about Urinary estrogens as a non-invasive biomarker of viable pregnancy in the giant panda (Ailuropoda melanoleuca).

Urinary specific gravity as an alternative for the normalisation of endocrine metabolite concentrations in giant panda (Ailuropoda melanoleuca) reproductive monitoring (2018)
Journal Article
Wauters, J., Wilson, K. S., Bouts, T., Valentine, I., Vanderschueren, K., Ververs, C., …Vanhaecke, L. (2018). Urinary specific gravity as an alternative for the normalisation of endocrine metabolite concentrations in giant panda (Ailuropoda melanoleuca) reproductive monitoring. PLOS ONE, 13(7), Article e0201420. https://doi.org/10.1371/journal.pone.0201420

Reproductive monitoring for captive breeding in giant pandas is based on behavioural observation and non-invasive hormone analysis. In urine, interpretation of results requires normalisation due to an animal’s changing hydration. Correction of urinar... Read More about Urinary specific gravity as an alternative for the normalisation of endocrine metabolite concentrations in giant panda (Ailuropoda melanoleuca) reproductive monitoring.

Preferential activation of HIF-2α adaptive signalling in neuronal-like cells in response to acute hypoxia (2017)
Journal Article
Martín-Aragón Baudel, M. A. S., Rae, M. T., Darlison, M. G., Poole, A. V., & Fraser, J. A. (2017). Preferential activation of HIF-2α adaptive signalling in neuronal-like cells in response to acute hypoxia. PLOS ONE, 12(10), Article e0185664. https://doi.org/10.1371/journal.pone.0185664

Stroke causes severe neuronal damage as disrupted cerebral blood flow starves neurons of oxygen and glucose. The hypoxia inducible factors (HIF-1α and HIF-2α) orchestrate oxygen homeostasis and regulate specific aspects of hypoxic adaptation. Here we... Read More about Preferential activation of HIF-2α adaptive signalling in neuronal-like cells in response to acute hypoxia.

Preferential activation of HIF-2 adaptive mechanisms in neuronal-like cells in response to hypoxia (2017)
Presentation / Conference
Fraser, J., Baudel, M., Rae, M., Darlison, M., & Poole, A. (2017, April). Preferential activation of HIF-2 adaptive mechanisms in neuronal-like cells in response to hypoxia. Poster presented at British Neuroscience Association - festival of neuroscience 2017

Stroke is a leading cause of death and disability worldwide. Blockage, or occlusion, of cerebral arteries causes irreversible neuronal damage as disrupted blood flow starves neurones of oxygen and glucose. The hypoxia inducible factors (HIFs) are mas... Read More about Preferential activation of HIF-2 adaptive mechanisms in neuronal-like cells in response to hypoxia.

The expression of the chloride co-transporters NKCC1 and KCC2 is reversed in the penumbra following (2017)
Conference Proceeding
Baudel, M., Rae, M., Fraser, J., & Poole, A. (2017). The expression of the chloride co-transporters NKCC1 and KCC2 is reversed in the penumbra following. In Brain and Neuroscience: BNA 2017 Festival of Neuroscience: Abstract Book

Stroke is one of the major causes of death and disability worldwide. The harm caused by the interruption of blood flow to the brain unfolds in the subsequent hours and days, so it is critical to identify new therapeutic targets that could reduce neur... Read More about The expression of the chloride co-transporters NKCC1 and KCC2 is reversed in the penumbra following.

Development of in vitro strategies to predict in vivo toxicity of engineered nanoparticles to the male reproductive system. (2016)
Presentation / Conference
Malone, E., Ross, B., Rae, M., Bouwmeester, H., Gosens, I., Cassee, F., …Hutchison, G. (2016, September). Development of in vitro strategies to predict in vivo toxicity of engineered nanoparticles to the male reproductive system. Poster presented at 11th International Particle Toxicology Conference

The introduction of engineered nanomaterials (ENM) to new materials and products requires informed risk assessment. The altered physico-chemical properties of ENM mean traditional assays to evaluate toxicity must be modified or validated to ensure... Read More about Development of in vitro strategies to predict in vivo toxicity of engineered nanoparticles to the male reproductive system..

Ovine trophoblasts express cathelicidin host defence peptide in response to infection (2016)
Journal Article
Coyle, C., Wheelhouse, N., Jacques, M., Longbottom, D., Svoboda, P., Pohl, J., …Barlow, P. G. (2016). Ovine trophoblasts express cathelicidin host defence peptide in response to infection. Journal of Reproductive Immunology, 117, 10-16. https://doi.org/10.1016/j.jri.2016.06.006

Cationic host defence peptides (CHDP; also known as antimicrobial peptides) are key components of the immune response in the female reproductive tract. The role of the placental trophoblast in ovine host defence remains poorly understood. This study... Read More about Ovine trophoblasts express cathelicidin host defence peptide in response to infection.

Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion (2016)
Journal Article
Ramaswamy, S., McNeilly, A. S., Rae, M. T., Ramaswamy, S., Grace, C., Mattei, A. A., …Rae, M. T. (2016). Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion. Scientific Reports, 6(1), Article 27408. https://doi.org/10.1038/srep27408

Exogenous androgenic steroids applied to pregnant sheep programmes a PCOS-like phenotype in female offspring. Via ultrasound guidance we applied steroids directly to ovine fetuses at d62 and d82 of gestation, and examined fetal (day 90 gestation) and... Read More about Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion.