All Outputs (14)

'ChatGPT & Me' Student Padlet Data With Reactions (2023)
Dataset
Drumm, L., Illingworth, S., Graham, C., Calabrese, P., Taylor, S., Dencer-Brown, I., & van Knippenberg, I. (2023). 'ChatGPT & Me' Student Padlet Data With Reactions. [Dataset]. https://doi.org/10.17869/enu.2023.3200728

These data are from a project which aimed to collect and analyse student attitudes to artificial intelligence (AI) and their own learning and assessment while they are studying with Edinburgh Napier University. AI tools such as ChatGPT make it easier... Read More about 'ChatGPT & Me' Student Padlet Data With Reactions.

Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection (2020)
Journal Article
Ho, J. S. Y., Angel, M., Ma, Y., Sloan, E., Wang, G., Martinez-Romero, C., …Marazzi, I. (2020). Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection. Cell, 181(7), 1502-1517. https://doi.org/10.1016/j.cell.2020.05.035

RNA viruses are a major human health threat. The life cycles of many highly pathogenic RNA viruses like influ-enza A virus (IAV) and Lassa virus depends on host mRNA, because viral polymerases cleave 50-m7G-cappedhost transcripts to prime viral mRNA... Read More about Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection.

Flexibility of bunyavirus genomes: creation of an orthobunyavirus with an ambisense S segment (2015)
Journal Article
van Knippenberg, I., & Elliott, R. (2015). Flexibility of bunyavirus genomes: creation of an orthobunyavirus with an ambisense S segment. Journal of Virology, 89(10), https://doi.org/10.1128/jvi.03595-14

The Bunyamwera (BUNV) orthobunyavirus NSs protein has proven a challenge to study in the context of viral infection. NSs is encoded in a reading frame that overlaps that of the viral nucleocapsid (N) protein thus limiting options for mutagenesis. In... Read More about Flexibility of bunyavirus genomes: creation of an orthobunyavirus with an ambisense S segment.

The transient nature of Bunyamwera orthobunyavirus NSs protein expression: effects of increased stability of NSs protein on virus replication (2013)
Journal Article
van Knippenberg, I., Fragkoudis, R., & Elliott, R. (2013). The transient nature of Bunyamwera orthobunyavirus NSs protein expression: effects of increased stability of NSs protein on virus replication. PLOS ONE, 8(5), Article e64137. https://doi.org/10.1371/journal.pone.0064137

The NSs proteins of bunyaviruses are the viral interferon antagonists, counteracting the host's antiviral response to infection. During high-multiplicity infection of cultured mammalian cells with Bunyamwera orthobunyavirus (BUNV), NSs is rapidly deg... Read More about The transient nature of Bunyamwera orthobunyavirus NSs protein expression: effects of increased stability of NSs protein on virus replication.

Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe (2013)
Journal Article
Elliott, R., Blakqori, G., van Knippenberg, I., Koudriakova, E., Li, P., McLees, A., …Szemiel, A. (2013). Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe. Journal of General Virology, 94(4), https://doi.org/10.1099/vir.0.049981-0

Schmallenberg virus (SBV) is a newly emerged orthobunyavirus that has caused widespread disease in cattle, sheep and goats in Europe. Like other orthobunyaviruses, SBV is characterized by a tripartite negative-sense RNA genome that encodes four struc... Read More about Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe.

The N-terminus of Bunyamwera orthobunyavirus NSs protein is essential for interferon antagonism (2010)
Journal Article
van Knippenberg, I., Carlton-Smith, C., & Elliott, R. (2010). The N-terminus of Bunyamwera orthobunyavirus NSs protein is essential for interferon antagonism. Journal of General Virology, 91(8), https://doi.org/10.1099/vir.0.021774-0

Bunyamwera virus NSs protein is involved in the inhibition of cellular transcription and the interferon (IFN) response, and it interacts with the Med8 component of Mediator. A spontaneous mutant of a recombinant NSs-deleted Bunyamwera virus (rBUNdelN... Read More about The N-terminus of Bunyamwera orthobunyavirus NSs protein is essential for interferon antagonism.

Rep provides a second motor at the replisome to promote duplication of protein-bound DNA (2009)
Journal Article
Guy, C., Atkinson, J., Gupta, M., Mahdi, A., Gwynn, E., Rudolph, C., …McGlynn, P. (2009). Rep provides a second motor at the replisome to promote duplication of protein-bound DNA. Molecular Cell, 36(4), 654-666. https://doi.org/10.1016/j.molcel.2009.11.009

Nucleoprotein complexes present challenges to genome stability by acting as potent blocks to replication. One attractive model of how such conflicts are resolved is direct targeting of blocked forks by helicases with the ability to displace the block... Read More about Rep provides a second motor at the replisome to promote duplication of protein-bound DNA.

Bunyamwera orthobunyavirus S-segment untranslated regions mediate poly(A) tail-independent translation (2009)
Journal Article
Blakqori, G., van Knippenberg, I., & Elliott, R. (2009). Bunyamwera orthobunyavirus S-segment untranslated regions mediate poly(A) tail-independent translation. Journal of Virology, 83(8), https://doi.org/10.1128/jvi.02201-08

The mRNAs of Bunyamwera virus (BUNV), the prototype of the Bunyaviridae family, possess a 5′ cap structure but lack a 3′ poly(A) tail, a common feature of eukaryotic mRNAs that greatly enhances translation efficiency. Viral mRNAs also contain untrans... Read More about Bunyamwera orthobunyavirus S-segment untranslated regions mediate poly(A) tail-independent translation.

Replication fork blockage by transcription factor-DNA complexes in Escherichia coli (2006)
Journal Article
Payne, B., van Knippenberg, I., Bell, H., Filipe, S., Sherratt, D., & McGlynn, P. (2006). Replication fork blockage by transcription factor-DNA complexes in Escherichia coli. Nucleic Acids Research, 34(18), 5194-5202. https://doi.org/10.1093/nar/gkl682

All organisms require mechanisms that resuscitate replication forks when they break down, reflecting the complex intracellular environments within which DNA replication occurs. Here we show that as few as three lac repressor-operator complexes block... Read More about Replication fork blockage by transcription factor-DNA complexes in Escherichia coli.

Tomato spotted wilt virus transcriptase in vitro displays a preference for cap donors with multiple base complementarity to the viral template (2005)
Journal Article
van Knippenberg, I., Lamine, M., Goldbach, R., & Kormelink, R. (2005). Tomato spotted wilt virus transcriptase in vitro displays a preference for cap donors with multiple base complementarity to the viral template. Virology, 335(1), 122-130. https://doi.org/10.1016/j.virol.2005.01.041

Transcription of segmented negative-strand RNA viruses is initiated by cap snatching: a host mRNA is cleaved generally at 10–20 nt from its 5′ capped end and the resulting capped leader used to prime viral transcription. For Tomato spotted wilt virus... Read More about Tomato spotted wilt virus transcriptase in vitro displays a preference for cap donors with multiple base complementarity to the viral template.

Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif (2005)
Journal Article
van Knippenberg, I., Goldbach, R., & Kormelink, R. (2005). Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif. Virus Research, 110(1-2), 125-131. https://doi.org/10.1016/j.virusres.2005.01.012

The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro tr... Read More about Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif.

In vitro transcription of Tomato spotted wilt virus is independent of translation (2004)
Journal Article
van Knippenberg, I., Goldbach, R., & Kormelink, R. (2004). In vitro transcription of Tomato spotted wilt virus is independent of translation. Journal of General Virology, 85(5), https://doi.org/10.1099/vir.0.19767-0

Ongoing transcription in vitro of Tomato spotted wilt virus (TSWV) has previously been demonstrated to require the presence of reticulocyte lysate. This dependence was further investigated by testing the occurrence of transcription in the presence of... Read More about In vitro transcription of Tomato spotted wilt virus is independent of translation.

Purified tomato spotted wilt virus particles support both genome replication and transcription in vitro (2002)
Journal Article
van Knippenberg, I., Goldbach, R., & Kormelink, R. (2002). Purified tomato spotted wilt virus particles support both genome replication and transcription in vitro. Virology, 303(2), 278-286. https://doi.org/10.1006/viro.2002.1632

Purified Tomato spotted wilt virus particles were shown to support either genome replication or transcription in vitro, depending on the conditions chosen. Transcriptional activity was observed only upon addition of rabbit reticulocyte lysate, indica... Read More about Purified tomato spotted wilt virus particles support both genome replication and transcription in vitro.

Characterization of the Escherichia coli damage-independent UvrBC endonuclease activity (1998)
Journal Article
Bazuine, M., van Knippenberg, I., Visse, R., & Goosen, N. (1998). Characterization of the Escherichia coli damage-independent UvrBC endonuclease activity. Journal of Biological Chemistry, 273(52), 34896-34903. https://doi.org/10.1074/jbc.273.52.34896

Incision of damaged DNA templates by UvrBC inEscherichia coli depends on UvrA, which loads UvrB on the site of the damage. A 50-base pair 3′ prenicked DNA substrate containing a cholesterol lesion is incised by UvrABC at two positions 5′ to the lesio... Read More about Characterization of the Escherichia coli damage-independent UvrBC endonuclease activity.