A. K.and Marshall Jones
Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor-like subunit with homology to the Drosophila melanogaster Dβ1 subunit
Jones, A. K.and Marshall; Marshall, A.K.Jones.J; Darlison, Mark; Blake, A.D.; Buckingham, S.D.; Darlison, M.G.; Sattelle, D.B.
Authors
A.K.Jones.J Marshall
Mark Darlison
A.D. Blake
S.D. Buckingham
M.G. Darlison
D.B. Sattelle
Abstract
The cloning, sequencing and functional expression of Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor (nAChR) subunit is described. This subunit shows 80% identity with the Drosophila melanogaster Dβ1 and 92% identity with the Locusta migratoria β1, non-α subunits but only 38% identity to Sgα1 (also referred to as αL1), a previously cloned S. gregaria nAChR α-subunit. When expressed in Xenopus laevis oocytes, Sgβ1 does not respond to nicotine. Responses to nicotine are observed, however, in oocytes co-expressing Sgα1 and Sgβ1, but the pharmacology is indistinguishable from that of currents produced by expressing Sgα1 alone. We conclude that either Sgβ1 does not co-assemble with Sgα1, or that it is unable to contribute to the functional properties of the receptor, in the Xenopus oocyte expression system.
Citation
Jones, A. K. M., Marshall, A., Darlison, M., Blake, A., Buckingham, S., Darlison, M., & Sattelle, D. (2005). Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor-like subunit with homology to the Drosophila melanogaster Dβ1 subunit. Invertebrate Neuroscience, 5(3-4), 147-155. https://doi.org/10.1007/s10158-005-0007-6
Journal Article Type | Article |
---|---|
Online Publication Date | Sep 22, 2005 |
Publication Date | 2005-11 |
Deposit Date | Jul 26, 2016 |
Journal | Invertebrate Neuroscience |
Print ISSN | 1354-2516 |
Electronic ISSN | 1439-1104 |
Publisher | BMC |
Peer Reviewed | Peer Reviewed |
Volume | 5 |
Issue | 3-4 |
Pages | 147-155 |
DOI | https://doi.org/10.1007/s10158-005-0007-6 |
Keywords | Nicotinic acetylcholine receptor, Locust (Schistocerca gregaria), Sgβ1 non-α subunit, Xenopus oocyte expression, Nicotinic receptor ligands |
Public URL | http://researchrepository.napier.ac.uk/Output/316463 |
Related Public URLs | http://dx.doi.org/10.1007/s10158-005-0007-6 |
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