Measurement of the Intracellular Mycobacterium tuberculosis Drug Effect and Prediction of the Clinical Dose–Response Relationship Using Intracellular Pharmacodynamic Modeling (PDi)

Abstract

The human disease tuberculosis (TB) is the leading cause of death from a single infectious agent. A quarter of the world’s population is estimated to be latently infected. Drug development and screening is slow and costly. We have developed a physiologically relevant assay to screen drugs against TB when inside immune cells. This chapter will describe a newly developed preclinical drug screening assay for TB, using high-content imaging and pharmacokinetic/pharmacodynamic modeling.

Citation

Donnellan, S., Martínez-Rodríguez, C., Aljayyoussi, G., & Biagini, G. A. (2021). Measurement of the Intracellular Mycobacterium tuberculosis Drug Effect and Prediction of the Clinical Dose–Response Relationship Using Intracellular Pharmacodynamic Modeling (PDi). In C. Barreiro, & J. Barredo (Eds.), Antimicrobial Therapies: Methods and Protocols (393-408). New York: Humana Press. https://doi.org/10.1007/978-1-0716-1358-0

• Acceptance Date: Jan 18, 2021
• Online Publication Date: May 12, 2021
• Publication Date: Jul 12, 2021
• Deposit Date: Feb 28, 2022
• Publisher: Humana Press
• Pages: 393-408
• Series Title: Methods in Molecular Biology book series
• Series Number: 2296
• Series ISSN: 1064-3745
• Book Title: Antimicrobial Therapies: Methods and Protocols
• ISBN: 978-1-0716-1357-3
• DOI: https://doi.org/10.1007/978-1-0716-1358-0
• Keywords: Tuberculosis, Drug screening, Mycobacteria, Antibiotics, Pharmacokinetic and pharmacodynamic drug Modeling
• Public URL: http://researchrepository.napier.ac.uk/Output/2849464
• Publisher URL: https://link.springer.com/protocol/10.1007/978-1-0716-1358-0_23