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Monitoring of drinking water quality using automated ATP quantification

Hansen, C.B.; Kerrouche, A.; Tatari, K.; Rasmussen, A.; Ryan, T.; Summersgill, P.; Desmulliez, M.P.Y.; Bridle, H.; Albrechtsen, H.J.

Authors

C.B. Hansen

K. Tatari

A. Rasmussen

T. Ryan

P. Summersgill

M.P.Y. Desmulliez

H. Bridle

H.J. Albrechtsen



Abstract

A microfluidic based system was developed for automated online method for the rapid detection and monitoring of drinking water contamination utilising microbial Adrenosine-5′-Triphosphate (ATP) as a bacterial indicator. The system comprises a polymethyl methacrylate based microfluidic cartridge inserted into an enclosure incorporating the functions of fluid storage and delivery, lysis steps and real-time detection. Design, integration and operation of the resulting automated system are reported, including the lysis method, the design of the mixing circuit, the choices of flow rate, temperature and reagent amount. Calibration curves of both total and free ATP were demonstrated to be highly linear over a range from 2.5–5000 pg/mL with the limit of detection being lower than 2.5 pg/mL of total ATP. The system was trialled in a lab study with different types of water, with lysis efficiency being found to be strongly dependent upon water type. Further development is required before online implementation.

Journal Article Type Article
Acceptance Date Aug 29, 2019
Online Publication Date Aug 30, 2019
Publication Date 2019-10
Deposit Date Sep 27, 2019
Publicly Available Date Aug 31, 2020
Journal Journal of Microbiological Methods
Print ISSN 0167-7012
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 165
Pages 105713
DOI https://doi.org/10.1016/j.mimet.2019.105713
Keywords Microfluidics, Drinking water, Microbial contamination, Monitoring, ATP
Public URL http://researchrepository.napier.ac.uk/Output/2149180

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