Monitoring of drinking water quality using automated ATP quantification

Hansen, C.B.; Kerrouche, A.; Tatari, K.; Rasmussen, A.; Ryan, T.; Summersgill, P.; Desmulliez, M.P.Y.; Bridle, H.; Albrechtsen, H.J.

doi:10.1016/j.mimet.2019.105713

Monitoring of drinking water quality using automated ATP quantification

Hansen, C.B.; Kerrouche, A.; Tatari, K.; Rasmussen, A.; Ryan, T.; Summersgill, P.; Desmulliez, M.P.Y.; Bridle, H.; Albrechtsen, H.J.

Authors

C.B. Hansen

Dr Abdelfateh Kerrouche A.Kerrouche@napier.ac.uk
Associate Professor

K. Tatari

A. Rasmussen

T. Ryan

P. Summersgill

M.P.Y. Desmulliez

H. Bridle

H.J. Albrechtsen

Abstract

A microfluidic based system was developed for automated online method for the rapid detection and monitoring of drinking water contamination utilising microbial Adrenosine-5′-Triphosphate (ATP) as a bacterial indicator. The system comprises a polymethyl methacrylate based microfluidic cartridge inserted into an enclosure incorporating the functions of fluid storage and delivery, lysis steps and real-time detection. Design, integration and operation of the resulting automated system are reported, including the lysis method, the design of the mixing circuit, the choices of flow rate, temperature and reagent amount. Calibration curves of both total and free ATP were demonstrated to be highly linear over a range from 2.5–5000 pg/mL with the limit of detection being lower than 2.5 pg/mL of total ATP. The system was trialled in a lab study with different types of water, with lysis efficiency being found to be strongly dependent upon water type. Further development is required before online implementation.

Citation

Hansen, C., Kerrouche, A., Tatari, K., Rasmussen, A., Ryan, T., Summersgill, P., Desmulliez, M., Bridle, H., & Albrechtsen, H. (2019). Monitoring of drinking water quality using automated ATP quantification. Journal of Microbiological Methods, 165, 105713. https://doi.org/10.1016/j.mimet.2019.105713

Journal Article Type	Article
Acceptance Date	Aug 29, 2019
Online Publication Date	Aug 30, 2019
Publication Date	2019-10
Deposit Date	Sep 27, 2019
Publicly Available Date	Aug 31, 2020
Journal	Journal of Microbiological Methods
Print ISSN	0167-7012
Publisher	Elsevier
Peer Reviewed	Peer Reviewed
Volume	165
Pages	105713
DOI	https://doi.org/10.1016/j.mimet.2019.105713
Keywords	Microfluidics, Drinking water, Microbial contamination, Monitoring, ATP
Public URL	http://researchrepository.napier.ac.uk/Output/2149180