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Phosphorylation of the epidermal growth factor
receptor (EGFR) is essential for interleukin-8
release from intestinal epithelial cells in response to
challenge with Escherichia coli O157 :H7 flagellin.

Fraser-Pitt, Douglas J; Cameron, Pamela; McNeilly, Tom N; Boyd, Amanda; Manson, Erin D T; Smith, David G E


Douglas J Fraser-Pitt

Pamela Cameron

Tom N McNeilly

Amanda Boyd

Erin D T Manson


Enterohaemorrhagic E. coli O157:H7 is a major food-borne and environmental pathogen responsible for both sporadic cases and outbreaks of food poisoning which can lead to serious sequelae, such as haemolytic uraemic syndrome. The structural subunit of E. coli O157:H7 flagella is flagellin, which is both the antigenic determinant of the H7 serotype, an important factor in colonisation, and an immunomodulatory protein determined to be a major pro-inflammatory component through the instigation of host cell signalling pathways. Flagellin has highly conserved N and C-terminal regions that are recognised by the host cell pattern recognition receptor, toll-like receptor 5 (TLR5). Activation of this receptor triggers cell signalling cascades, which are known to activate host cell kinases and transcription factors that respond with the production of inflammatory mediators such as the chemokine interleukin-8 (IL-8), although the exact components of this pathway are not yet fully characterised. We demonstrate that E. coli O157:H7-derived flagellin induces rapid phosphorylation of the EGFR, as an early event in intestinal epithelial cell signalling, and that this is required for the release of pro-inflammatory cytokine IL-8.


Fraser-Pitt, D. J., Cameron, P., McNeilly, T. N., Boyd, A., Manson, E. D. T., & Smith, D. G. E. (2011). challenge with Escherichia coli O157 :H7 flagellin. Microbiology, 157, 2339-2347.

Journal Article Type Article
Acceptance Date May 3, 2011
Publication Date 2011-05
Deposit Date Jun 22, 2012
Print ISSN 1350-0872
Electronic ISSN 1465-2080
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 157
Pages 2339-2347
Keywords EHEC; E. coli O157:H7; flagellin; EGFR; TLR5; IL-8;
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