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Sleep disruption and its effect on lymphocyte redeployment following an acute bout of exercise

Florida-James, Geraint D.; Ingram, Lesley A.; Simpson, Richard J.; Malone, Eva; Florida-James, Geraint


Geraint D. Florida-James

Richard J. Simpson


Sleep disruption and deprivation are common in contemporary society and have been linked with poor health, decreased job performance and increased life-stress. The rapid redeployment of lymphocytes between the blood and tissues is an archetypal feature of the acute stress response, but it is not known if short-term perturbations in sleep architecture affect lymphocyte redeployment. We examined the effects of a disrupted night sleep on the exercise-induced redeployment of lymphocytes and their subtypes. 10 healthy male cyclists performed 1 h of cycling at a fixed power output on an indoor cycle ergometer, following a night of undisrupted sleep (US) or a night of disrupted sleep (DS). Blood was collected before, immediately after and 1 h after exercise completion. Lymphocytes and their subtypes were enumerated using direct immunofluorescence assays and 4-colour flow cytometry. DS was associated with elevated concentrations of total lymphocytes and CD3−/CD56+ NK-cells. Although not affecting baseline levels, DS augmented the exercise-induced redeployment of CD8+ T-cells, with the naïve/early differentiated subtypes (KLRG1−/CD45RA+) being affected most. While the mobilisation of cytotoxic lymphocyte subsets (NK cells, CD8+ T-cells γδ T-cells), tended to be larger in response to exercise following DS, their enhanced egress at 1 h post-exercise was more marked. This occurred despite similar serum cortisol and catecholamine levels between the US and DS trials. NK-cells redeployed with exercise after DS retained their expression of perforin and Granzyme-B indicating that DS did not affect NK-cell ‘arming’. Our findings indicate that short-term changes in sleep architecture may ‘prime’ the immune system and cause minor enhancements in lymphocyte trafficking in response to acute dynamic exercise.

Journal Article Type Article
Acceptance Date Dec 15, 2014
Online Publication Date Jan 9, 2015
Publication Date 2015-07
Deposit Date Jan 15, 2015
Journal Brain, Behavior and Immunity
Print ISSN 0889-1591
Electronic ISSN 1090-2139
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 47
Pages 100-108
Keywords Sleep disruption; sleep deprivation; Cytotoxic lymphocytes; immune system;
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