Responses of saccharomyces cerevisiae strains from different origins to elevated iron concentrations.

Mart�nez-Garay, Carlos Andr�s; Romero, Antonia Mar�a; Mart�nez-Pastor, Mar�a Teresa; de Llanos, Rosa; Puig, Sergi

Abstract

Iron is an essential micronutrient for all eukaryotic organisms. However, the low solubility of ferric iron has tremendously increased the prevalence of iron deficiency anemia, especially in women and children, with dramatic consequences. Baker's yeast Saccharomyces cerevisiae is used as a model eukaryotic organism, a fermentative microorganism, and a feed supplement. In this report, we explore the genetic diversity of 123 wild and domestic strains of S. cerevisiae isolated from different geographical origins and sources to characterize how yeast cells respond to elevated iron concentrations in the environment. By using two different forms of iron, we selected and characterized both iron-sensitive and iron-resistant yeast strains. We observed that when the iron concentration in the medium increases, iron-sensitive strains accumulate iron more rapidly than iron-resistant isolates. We observed that, consistent with excess iron leading to oxidative stress, the redox state of iron-sensitive strains was more oxidized than that of iron-resistant strains. Growth assays in the presence of different oxidative reagents ruled out that this phenotype was due to alterations in the general oxidative stress protection machinery. It was noteworthy that iron-resistant strains were more sensitive to iron deficiency conditions than iron-sensitive strains, which suggests that adaptation to either high or low iron is detrimental for the opposite condition. An initial gene expression analysis suggested that alterations in iron homeostasis genes could contribute to the different responses of distant iron-sensitive and iron-resistant yeast strains to elevated environmental iron levels.

Citation

Martínez-Garay, C. A., Romero, A. M., Martínez-Pastor, M. T., de Llanos, R., & Puig, S. (2016). Responses of saccharomyces cerevisiae strains from different origins to elevated iron concentrations. Applied and Environmental Microbiology, 82(6), 1906-1916. https://doi.org/10.1128/AEM.03464-15

• Journal Article Type: Article
• Acceptance Date: Jan 8, 2016
• Online Publication Date: Jan 15, 2016
• Publication Date: Jan 15, 2016
• Deposit Date: Mar 16, 2016
• Publicly Available Date: Mar 16, 2016
• Print ISSN: 0099-2240
• Electronic ISSN: 1098-5336
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 82
• Issue: 6
• Pages: 1906-1916
• DOI: https://doi.org/10.1128/AEM.03464-15
• Keywords: Iron; Anaemia; Environmental
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/9665
• Publisher URL: http://dx.doi.org/10.1128/AEM.03464-15