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Responses of saccharomyces cerevisiae strains from different origins to elevated iron concentrations.

Mart�nez-Garay, Carlos Andr�s; Romero, Antonia Mar�a; Mart�nez-Pastor, Mar�a Teresa; de Llanos, Rosa; Puig, Sergi


Carlos Andr�s Mart�nez-Garay

Antonia Mar�a Romero

Mar�a Teresa Mart�nez-Pastor

Rosa de Llanos

Sergi Puig


A. A. Brakhage


Iron is an essential micronutrient for all eukaryotic organisms. However, the low solubility of ferric iron has tremendously increased the prevalence of iron deficiency anemia, especially in women and children, with dramatic consequences. Baker's yeast Saccharomyces cerevisiae is used as a model eukaryotic organism, a fermentative microorganism, and a feed supplement. In this report, we explore the genetic diversity of 123 wild and domestic strains of S. cerevisiae isolated from different geographical origins and sources to characterize how yeast cells respond to elevated iron concentrations in the environment. By using two different forms of iron, we selected and characterized both iron-sensitive and iron-resistant yeast strains. We observed that when the iron concentration in the medium increases, iron-sensitive strains accumulate iron more rapidly than iron-resistant isolates. We observed that, consistent with excess iron leading to oxidative stress, the redox state of iron-sensitive strains was more oxidized than that of iron-resistant strains. Growth assays in the presence of different oxidative reagents ruled out that this phenotype was due to alterations in the general oxidative stress protection machinery. It was noteworthy that iron-resistant strains were more sensitive to iron deficiency conditions than iron-sensitive strains, which suggests that adaptation to either high or low iron is detrimental for the opposite condition. An initial gene expression analysis suggested that alterations in iron homeostasis genes could contribute to the different responses of distant iron-sensitive and iron-resistant yeast strains to elevated environmental iron levels.

Journal Article Type Article
Acceptance Date Jan 8, 2016
Online Publication Date Jan 15, 2016
Publication Date Jan 15, 2016
Deposit Date Mar 16, 2016
Publicly Available Date Mar 16, 2016
Print ISSN 0099-2240
Electronic ISSN 1098-5336
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 82
Issue 6
Pages 1906-1916
Keywords Iron; Anaemia; Environmental;
Public URL
Publisher URL
Contract Date Mar 16, 2016


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