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Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes (1997)
Journal Article
Harvey, R. J., Stühmer, T., van Minnen, J., & Darlison, M. G. (1997). Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes. Neuroscience Research Communications, 20(1), 31-40. https://doi.org/10.1002/%28SICI%291520-6769%28199701%2920%3A13.0.CO%3B2-6

We report the isolation of molluscan (Lymnaea stagnalis) partial complementary DNAs (cDNAs) that encode two new putative glutamate-gated cation-channel polypeptides. These proteins, which we have named Lym-eGluR4 and Lym-eGluR5, exhibit 56% identity... Read More about Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes.

Biosynthesis of the glycolipid anchor of lipophosphoglycan and the structurally related glycoinositolphospholipids from Leishmania major (1995)
Journal Article
Proudfoot, L., Schneider, P., Ferguson, M. A. J., & McConville, M. J. (1995). Biosynthesis of the glycolipid anchor of lipophosphoglycan and the structurally related glycoinositolphospholipids from Leishmania major. The biochemical journal, 308(1), 45-55. https://doi.org/10.1042/bj3080045

The major macromolecule on the surface of the protozoan parasite Leishmania major is a lipophosphoglycan (LPG) which contains a glycosylphosphatidylinositol glycolipid anchor. This parasite also synthesizes a complex family of abundant low-molecular-... Read More about Biosynthesis of the glycolipid anchor of lipophosphoglycan and the structurally related glycoinositolphospholipids from Leishmania major.

GABAAreceptor subtypes: which, where and why? (1995)
Journal Article
Darlison, M. G., & Albrecht, B. E. (1995). GABAAreceptor subtypes: which, where and why?. Seminars in the neurosciences, 7(2), 115-126. https://doi.org/10.1006/smns.1995.0013

Synaptic inhibition in the vertebrate central nervous system is mediated predominantly by subtypes of the GABAAreceptor, which comprise different pentameric combinations of the products of 13 genes. In this article, we review the results of recent ex... Read More about GABAAreceptor subtypes: which, where and why?.

Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis (1994)
Journal Article
Hicks, A. A., Bailey, M. E., Riley, B. P., Kamphuis, W., Siciliano, M. J., Johnson, K. J., & Darlison, M. G. (1994). Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis. Genomics, 20(2), 285-288. https://doi.org/10.1006/geno.1994.1167

GABAA receptors are hetero-oligomeric ion-channel complexes that are composed of combinations of α, β, γ, and δ subunits and play a major role in inhibitory neurotransmission in the mammalian brain. We report here a microsatellite polymorphism within... Read More about Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis.

Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique (1993)
Journal Article
Lasham, A., & Darlison, M. G. (1993). Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique. Molecular and Cellular Probes, 7(1), 67-73. doi:10.1006/mcpr.1993.1009

We described an improved method for directly sequencing lambda (λ) DNA that has been isolated from either crude cleared lysates or plate lysates. This protocol does not require that the DNA be obtained from bacteriophage particles that have been puri... Read More about Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique.

Confirmation of the localization of the human GABAA receptor α1-subunit gene (GABRA1) to distal 5q by linkage analysis (1992)
Journal Article
Johnson, K. J., Sander, T., Hicks, A. A., van Marle, A., Janz, D., Mullan, M. J., …Darlison, M. G. (1992). Confirmation of the localization of the human GABAA receptor α1-subunit gene (GABRA1) to distal 5q by linkage analysis. Genomics, 14(3), 745-748. https://doi.org/10.1016/s0888-7543%2805%2980178-8

The GABAA receptor is the major inhibitory neurotransmitter receptor in the mammalian brain. To date, 14 genes that encode subunits of this receptor have been identified; these appear to be scattered throughout the human genome and are under investig... Read More about Confirmation of the localization of the human GABAA receptor α1-subunit gene (GABRA1) to distal 5q by linkage analysis.

Distribution of the GABAA receptor α1- and γ2-subunit mRNAs in chick brain (1991)
Journal Article
Glencorse, T. A., Bateson, A. N., Hunt, S. P., & Darlison, M. G. (1991). Distribution of the GABAA receptor α1- and γ2-subunit mRNAs in chick brain. Neuroscience Letters, 133(1), 45-48. https://doi.org/10.1016/0304-3940%2891%2990053-v

We have used sequence-specific oligonucleotide probes and in situ hybridisation histochemistry to examine the distributions of the GABAA receptor α1- and γ2-subunit mRNAs in serial sections of 1-day-old chick brain. Both transcripts are present toget... Read More about Distribution of the GABAA receptor α1- and γ2-subunit mRNAs in chick brain.

The surface lipid of parasitic nematodes: Organization, and modifications during transition to the mammalian host environment (1990)
Journal Article
Proudfoot, L., Kusel, J. R., Smith, H. V., Harnett, W., Worms, M. J., & Kennedy, M. W. (1990). The surface lipid of parasitic nematodes: Organization, and modifications during transition to the mammalian host environment. Acta tropica, 47(5-6), 323-330. https://doi.org/10.1016/0001-706x%2890%2990033-v

The biophysical properties of the surface lipid of a range of nematode species and their developmental stages were examined, using fluorescent lipid probes and fluorescence recovery after photobleaching (FRAP). These methods can be applied to living,... Read More about The surface lipid of parasitic nematodes: Organization, and modifications during transition to the mammalian host environment.

Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli (1984)
Journal Article
Wood, D., Darlison, M. G., Wilde, R. J., & Guest, J. R. (1984). Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli. The biochemical journal, 222(2), 519-534. https://doi.org/10.1042/bj2220519

The nucleotide sequence of a 3614 base-pair segment of DNA containing the sdhA gene, encoding the flavoprotein subunit of succinate dehydrogenase of Escherichia coli, and two genes sdhC and sdhD, encoding small hydrophobic subunits, has been determin... Read More about Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli.

Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase (1984)
Journal Article
Spencer, M. E., Darlison, M. G., Stephens, P. E., Duckenfield, I. K., & Guest, J. R. (1984). Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase. European journal of biochemistry / FEBS, 141(2), 361-374. https://doi.org/10.1111/j.1432-1033.1984.tb08200.x

The nucleotide sequence of the sucB gene, which encodes the dihydrolipoamide succinyltransferase component (E2o) of the 2‐oxoglutarate dehydrogenase complex of Escherichia coli K12, has been determined by the dideoxy chain‐termination method. The res... Read More about Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase.

Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12 (1984)
Journal Article
Darlison, M. G., Spencer, M. E., & Guest, J. R. (1984). Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12. European journal of biochemistry / FEBS, 141(2), 351-359. https://doi.org/10.1111/j.1432-1033.1984.tb08199.x

The nucleotide sequence of a 3180‐base‐pair segment of DNA, containing the sucA gene encoding the 2‐oxoglutarate dehydrogenase component (E1o) of the 2‐oxoglutarate dehydrogenase complex of Escherichia coli, has been determined by the dideoxy chain‐t... Read More about Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12.

Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12 (1983)
Journal Article
Stephens, P. E., Lewis, H. M., Darlison, M. G., & Guest, J. R. (1983). Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12. European journal of biochemistry / FEBS, 135(3), 519-527. https://doi.org/10.1111/j.1432-1033.1983.tb07683.x

The nucleotide sequence of a 1980‐base‐pair segment of DNA, containing the lpd gene encoding the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase complex of Escherichia coli K 12, has been determined by the dideoxy chain‐terminati... Read More about Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12.

The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Dihydrolipoamide Acetyltransferase Component (1983)
Journal Article
Stephens, P. E., Darlison, M. G., Lewis, H. M., & Guest, J. R. (1983). The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Dihydrolipoamide Acetyltransferase Component. European journal of biochemistry / FEBS, 133(3), 481-489. https://doi.org/10.1111/j.1432-1033.1983.tb07490.x

The nucleotide sequence of the aceF gene, which encodes the dihydrolipoamide acetyltransferase component (E2) of the pyruvate dehydrogenase complex of Escherichia coli K12, has been determined using the dideoxy chain‐termination method. The aceF gene... Read More about The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Dihydrolipoamide Acetyltransferase Component.

The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Pyruvate Dehydrogenase Component (1983)
Journal Article
Stephens, P. E., Darlison, M. G., Lewis, H. M., & Guest, J. R. (1983). The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Pyruvate Dehydrogenase Component. European journal of biochemistry / FEBS, 133(1), 155-162. https://doi.org/10.1111/j.1432-1033.1983.tb07441.x

The nucleotide sequence of a 3780‐base pair segment of DNA containing the aceE gene encoding the pyruvate dehydrogenase component (E1) of the pyruvate dehydrogenase complex of Escherichia coli, has been determined by the dideoxy chain‐termination met... Read More about The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Pyruvate Dehydrogenase Component.

Genetics of oxidative phosphorylation: Allelism studies of mitochondrial loci in the PHO1?OLI2 region of the genome (1980)
Journal Article
Darlison, M. G., & Lancashire, W. E. (1980). Genetics of oxidative phosphorylation: Allelism studies of mitochondrial loci in the PHO1?OLI2 region of the genome. Molecular & general genetics : MGG, 180(1), 227-229. https://doi.org/10.1007/bf00267374

In a search for new aerobic-growth deficiency mutations affecting mitochondrial energy-conservation two mit− mutations, namely pho-8 and pho-9, have been isolated. The two mutations are allelic with each other, but not allelic with the previously kn... Read More about Genetics of oxidative phosphorylation: Allelism studies of mitochondrial loci in the PHO1?OLI2 region of the genome.